Ciprofloxacin is a second generic and an antibiotic generic drug. It is a very popular fluoroquinolone that can treat a number of bacterial infections. The quinolones are family or a group that has activity against both-negative and positive-gram bacteria, when compared to Ciprofloxacin. The first aim of this review is to provide a comprehensive update of analytical methods for determining the Ciprofloxacin and its combinations, binary or triple mixture use of endodontic treatment of primary teeth using a combination of antibacterial drugs. The other aim of this review is to evaluate clinical and radiographic success of the endodontic treatment of infected primary teeth using a combination of ciprofloxacin, metronidazole, minocycline, and clindamycin. Several methods were used in the analytical determination of Ciprofloxacin and its metabolites in human biological fluids (plasma) and in pharmaceutical preparations.